For students
Research
Publications
Coworkers
Service
FTIR spectra libraries
Master collection







Zentralinstitut für Ernährungs- und Lebensmittelforschung (ZIEL)

Abteilung Mikrobiologie
Technische Universität München
Weihenstephaner Berg 3
D-85350 Freising


Food Microbiol. 2016 Feb;53(Pt B):15-23. doi: 10.1016/j.fm.2015.07.017. Epub 2015 Aug 15.

Spoilage of vacuum-packed beef by the yeast Kazachstania psychrophila.
Kabisch J1, Erl-Höning C2, Wenning M3, Böhnlein C2, Gareis M4, Pichner R2.

Abstract
A survey of the psychrotolerant yeast microbiota of vacuum-packed beef was conducted between 2010 and 2012. Chilled vacuum-packed beef (n = 50) sampled from 15 different producers was found to have a mean psychrotolerant yeast count of 3.76 log cfu per cm2. During this assessment, a recently described yeast named Kazachstania psychrophila was shown to be associated with this product. In order to gain basic knowledge about the spoilage potential of K. psychrophila in vacuum-packed beef, challenge studies were performed and the survival of three different K. psychrophila strains was analyzed during storage of artificially contaminated beef. Beef samples were inoculated with the yeasts at a contamination level of 2 log cfu per cm2. Survival and growth of K. psychrophila strains was monitored on malt extract agar at regular intervals over 84 days. Kazachstania levels rapidly increased about 5 log units within 16 days under chill conditions (4 °C). Gas bubbles were observed after 16 days, while discoloration and production of off-flavors became evident after 42 days in inoculated samples. This study demonstrates for the first time, that the psychrotolerant yeast K. psychrophila is a dominant spoilage microorganism of vacuum-packed beef products stored at low temperatures, causing sensory defects which result in reduced shelf life, and consequently in considerable economic losses.
Copyright © 2015 Elsevier Ltd. All rights reserved.

KEYWORDS:
Beef microbiota; Food; Meat spoilage; Saccharomycetaceae


News

Phd student position
PhD student position in molecular microbiology


Innovationspreis für Siegfried Scherer


Otto-von-Guericke Forschungspreis für Siegfried Scherer

Paper

Paper
Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq-ryhB encodes the regulatory RNA RyhB and a Peptide, RyhP. (2017)

Paper
Complete circular genome sequence and temperature independent adaptation to Anaerobiosis of Listeria weihenstephanensis DSM 24698 (2017)

Paper
Discovery of numerous novel small genes in the intergenic regions of the Escherichia coli O157:H7 Sakai Genome (2017)

Paper
High binding affinity of repressor IolR avoids costs of untimely induction of myo-inositol utilization by Salmonella Typhimurium (2017)

Paper
Genetic characterization of the Galactitol utilization pathway of Salmonella enterica serovar Typhimurium. (2017)

Paper
Heat stability of indigenous milk plasmin and peptidases from Pseudomonas: a challenge in the production of ultra-high temperature milk products (2016)

Paper
Spoilage of Microfiltered and Pasteurized Extended Shelf Life Milk Is Mainly Induced by Psychrotolerant Spore-Forming Bacteria that often Originate from Recontamination (2017)

Paper
Bakterieller Stoffwechsel als Pathogenitätsfaktor (2016)

Paper
Transcriptional and translational regulation by RNA thermometers, riboswitches and the sRNA DsrA in Escherichia coli O157:H7 Sakai under combined cold and osmotic stress adaptation. (2016)

Paper
Hysteresis in myo-inositol utilization by Salmonella Typhimurium (2016)

Paper
Probiotic Enterococcus faecalis Symbioflor® down regulates virulence genes of EHEC in vitro and decrease pathogenicity in a Caenorhabditis elegans model (2017)

Paper
Draft Genome Sequence of Lysinibacillus xylanilyticus SR-86 (Nov. 2016)

Paper
Permanent colonization of creek sediments, creek water and limnic water plants by four Listeria species in low population densities (November 2016)

Paper
Draft Genome Sequence of the Xanthan Producer Xanthomonas campestris LMG 8031 (Oktober 2016)

Paper
Comparative Bioinformatics and Experimental Analysis of the Intergenic Regulatory Regions of Bacillus cereus hbl and nhe Enterotoxin Operons and the Impact of CodY on Virulence Heterogeneity (Mai 2016)

Paper
Regulation of fucose and 1,2-propanediol utilization by Salmonella enterica serovar Typhimurium (März 2016)

Paper
Acidified nitrite inhibits proliferation of Listeria monocytogenes - Transcriptional analysis of a preservation method (März 2016)



Paper
Translatomics combined with transcriptomics and proteomics reveals novel functional, recently evolved orphan genes in Escherichia coli O157:H7 (EHEC)

Paper
A sensitive and robust method for direct determination of lipolytic activity in natural milk environment (März 2016, online first)

Paper
Evidence for the recent origin of a bacterial protein-coding, overlapping orphan gene by evolutionary overprinting (Dez. 2015)

Paper
Massive horizontal gene transfer, strictly vertical inheritance and ancient duplications differentially shape the evolution of Bacillus cereus enterotoxin operons hbl, cytK and nhe (Nov. 2015)

Paper
Chemodiversity of cereulide, the emetic Toxin of Bacillus cereus (2015)